Fronting peaks …

fronting peak

In HPLC, Fronting peaks are as common as tailing peaks.

Many analysts believe that sample overload causes peak tailing. They’re wrong.

Sample overload causes peak fronting.

If you have fronting peaks, don’t worry. Your column is fine. These are the reasons:

  • Sample overload
    • Reduce sample concentration.
    • Reduce sample volume.
  • Unresolved peak on the front
    • Change detection wavelength, or if you have one, use a diode-array detector.
    • Change mobile phase. Specifically, reduce the stronger solvent a bit.
  • Sample solvent incompatible with mobile phase
    • Dissolve sample in mobile phase.

Got it?

SKS

Advertisements