Peaks with tails …

tailing peak

Tailing peaks are a nuisance in HPLC. And they happen all the time.

The common misconception about peak tailing is that it is caused by sample overload. No, it’s not.

Most of the time, peak tailing is caused by a bad column.

Here’s why peaks tail, and what to do about it:

1) Active silanol sites on column – Use 0.1 % triethylamine in mobile phase. Or get a better column, with a higher carbon load.

2 ) Sample ionisation – Adjust pH to suppress ionisation.

3) K’ too large – Increase mobile phase strength.

4) Insufficient end-capping – Change column.

5) Hidden peak on tail – Change detection wavelength and/or change mobile phase strength.

If you have persistent peak tailing, you have a bad column. You have no choice but to change it.

Cheers … SKS

 

 

 

Advertisements

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out /  Change )

Google+ photo

You are commenting using your Google+ account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )

Connecting to %s